In vivo Biochemical Evaluation of Some Synthesize Thiazole Derivatives Containing Coumarin Moiety as Antioxidant and Antitumor Agents
F. Z. Mohammed
Department of Biochemistry, Faculty of Science, Zagazig University, Egypt.
Lamiaa A. A. Barakat
Department of Biochemistry, Faculty of Science, Port-Said University, Egypt.
Emad M. Gad
Department of Chemistry, Faculty of Science, Suez Canal University, Egypt.
N. M. Shetya *
Department of Biochemistry, Faculty of Science, Suez Canal University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Background: Coumarin and thiazole derivatives have been used traditionally for many centuries. Because they have anti-tumor, antioxidant activities, and induced apoptosis.
Aim: The present study aims to investigate the in vivo antitumor, and antioxidant activities of potassium salt of 5-(4 -chlorophenyl)-2-[(7-hydroxy coumarin-3-ylethylidene) acetyl hydrazine]-1,3 thiazole (5a) and 5-(4 -chlorophenyl)-2-[(8-hydroxy coumarin-3-ylethylidene) acetyl hydrazine]-1,3 thiazole (5b).
Materials and Methods: Toxicity has been determined for the synthesized compound. Anti-cancer and anti-oxidant activities were studied through the evaluation of tumor cell viability, lifespan prolongation and antioxidant estimation.
Results: Doses of up to 500 mg/kg showed good protection in 5a and 5b compounds. Results of in vivo anti-tumor activity against Ehrlich ascites carcinoma (EAC) cells for the compounds studied showed a significant reduction in the volume and number of ascites. And increased in life span. In therapeutic and preventive groups, compounds 5a and 5b have anti-oxidant properties by a significant decrease in malondialdehyde and significant increased catalase and GSH. Also, compounds 5a and 5b induced apoptosis by increase Caspase-3 and BCL-2 associated protein (BAX), Compared to the group of positive controls.
Conclusion: It was concluded that compounds 5a and 5b have a potent antitumor and antioxidant activity against Ehrlich ascites carcinoma in mice.
Keywords: Thiazoles, cytotoxicity, antioxidant, antitumor, Ehrlich ascites carcinoma, in vivo.