Anti-cancer Activity of Garcinia mangostana L. and Its Derivatives in Cervical Cancer
S. V. Kirthanashri
Amity Institute of Molecular Medicine and Stem Cell Research, Amity University, Sector - 125, Gautam Buddha Nagar Noida - 201313, Uttar Pradesh, India.
N. Ramesh Kumar
Department of Pharmaceutical Chemistry, C. L. Baid Metha College of Pharmacy, Thorapakkam, Chennai-600097, India.
S. Chitra *
Captain Srinivasa Murthy Regional Ayurveda Drug Development Institute, Central Council for Research in Ayurvedic Sciences, M/o AYUSH Govt. of India, A. A. Hospital Campus, Arumbakkam, Chennai-600106, India.
*Author to whom correspondence should be addressed.
Abstract
The Garcinia mangostana Linn, a medicinal plant commonly used in Southeast Asia. The crude extract and isolated metabolites were used to evaluate its potential anti-cancer activity which was compared to methotrexate (MTX) in cervical cancer cell line. The present study involved in using crude mangosteen (CM), γ-mangostin (γ-M), isopropyl mangostin (IPM) and Di-O-methyl mangostin (DMM) against the standard anti-neoplastic drug MTX. Cell viability and cytotoxicity by lactate dehydrogenase (LDH) were assessed. Analysis of DNA fragmentation and clonogenic assay further supports the anti-cancer activity of the drugs in HeLa cells. The IC50 value for CM, γ-M, DMM, IPM and MTX were 13.4µg, 34.84µM, 15.57µM 5.3µM and 16.05µM respectively observed in the present study. This study suggests that the G. mangostana and its derivatives have potential anti-cancer activity an in vitro study in cervical cancer.
Keywords: Cervical cancer, Garcinia mangostana, DNA fragmentation, MTT, clonogenic assay, cytotoxicity.