Purification and Characterization of Superoxide Dismutase Enzyme from Punica granatum L.
Caglar Ozdemir
University of Cukurova, Art and Science Faculty, Chemistry Department (Biochemistry Division) 01330, Balcali, Adana, Turkey.
Nuri Gulesci *
Gumushane University, Gumushane Vocational School, Department of Chemistry and Chemical Processing Technologies, 29100 Gumushane, Turkey.
Ramazan Bilgin
University of Cukurova, Art and Science Faculty, Chemistry Department (Biochemistry Division) 01330, Balcali, Adana, Turkey.
*Author to whom correspondence should be addressed.
Abstract
Superoxide dismutase (SOD) constitutes a very important antioxidant defense against oxidative stress in the body. SOD is found aplenty in many organisms, from microorganisms to plants and animals. Pomegranate (Punica granatum L.) peels and fruits have been used in traditional medicine. In this study, SOD (E.C 1.15.1.1) was purified from Punica granatum L. For this purpose, Punica granatum L. was homogenized centrifuged, fractioned with ammonium sulfate to precipitate, and then DEAE chromatography separation was applied. Punica granatum L. was purified 16.60-fold. SOD enzyme-specific activity was found as 166 U/mg protein.
Keywords: Punica granatum L., pomegranate, purification, superoxide dismutase