Regeneration of Pancreas Β-Cells in Alloxan-Induced Diabetes Rats Treated with Aqueous Seed Extract of Syzygium aromaticum: A Preliminary Study
Azeemat T. Abdulazeez
Biological Sciences Department, Faculty of Natural and Applied Sciences, Al-Hikmah University, Ilorin 240243, Nigeria.
Kehinde H. Bello
Biological Sciences Department, Faculty of Natural and Applied Sciences, Al-Hikmah University, Ilorin 240243, Nigeria.
Mutiu A. Alabi *
Medical Biochemistry and Pharmacology Department, College of Pure and Applied Sciences, Kwara State University, Malete 241104, Nigeria.
Fatai A. Kareem
Department of Pharmaceutical Science, School of Science and Technology, Gateway Polytechnic, Saapade 121104, Nigeria.
Janet F. Adeegbe
Bioresources Development Centre, National Biotechnology Development Agency, Ogbomoso 210222, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: The present study was conducted to investigate the preventive/curative effect of aqueous seed extract Syzygium aromaticum in alloxan-induced diabetes in rats.
Study Design: Twenty Wistar rats were randomly divided into four groups. The first group served as a control (received 1 ml of normal saline). The second group served as Diabetic control (received 1 ml of normal saline). The third group served as test 1 (pre-treated with 500 mg/kg body weight (b.w.) of aqueous Syzygium aromaticum seeds extract for seven days prior to diabetic induction and post-treated with aqueous seeds extract of Syzygium aromaticum after diabetic induction) and the fourth group served as test 2 (post-treated with 500 mg/kg b.w. of aqueous seed extract of Syzygium aromaticum after diabetic induction) in a daily oral dose for 14 days.
Results: Treatment of rats with aqueous seed extract of Syzygium aromaticum in a daily dose of 500 mg/kg significantly mitigates the induced changes in the glucose and lipid profile parameters. Histopathological examination of the pancreas showed the destruction of β-cells in the diabetic control group and recovery of damaged tissues when treated with 500 mg/kg b.w. of aqueous seeds extract of Syzygium aromaticum.
Conclusion: The present study suggests that aqueous seed extract S. aromaticum seeds extract at a dose of 500 mg/kg body weight brings about regeneration of the B-cells of the pancreas and significant beneficial effects in various physiological parameters in alloxan-induced diabetes.
Keywords: Syzygium aromaticum, diabetes, alloxan, blood glucose, pancreas