Membrane Stabilization, PhospholipaseA2, Albumin Denaturation, Protease Inhibition, as Viable Mechanisms for the Anti-Inflammatory Effects of Methanol Extract of Rauvolfia vomitoria Afzel Leaves
Ugwu Obiora Celestine *
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria and Department of Biochemistry, Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria.
Asogwa Felix Keneolisa
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria.
Ukpabi-Ugo Jacinta Chigozie
Department of Biochemistry, College of Natural Science, Micheal Okpara University of Agriculture, Umudike, Abia State, Nigeria.
Asadu Chidimma Lilian
Department of Medical Biochemistry, College of Medicine, Enugu State University of Science and Technology, Parklane, Enugu, Nigeria.
Ali Ibeabuchi Jude
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria.
Okonkwo Raymond Maduabuchi
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria.
Ugwoke Faith Ifeanyi
Uburu Bioresources Biotechnology Development Agency, Abuja, Nigeria.
Offiah Raymond Ogbonna
Department of Pharmacology and Therapeutics, Faculty of Clinical Medicine, Enugu State University of Science and Technology, Nigeria.
Obasi Uchenna Emmanuel
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria.
Anosike Chioma Assumpta
Department of Biochemistry, Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Background: The body uses inflammation as a defence mechanism to eliminate harmful stimuli like germs, damaged cells, or irritants and to initiate the healing process. However, the ongoing discovery of several medicinal plants and the testing of their bioactivity to produce information that will assist doctors and patients in making informed decisions prior to employing them have established.
Aims: The study valuate the phosphatelipase A2, membrane stabilization, albumin denaturation, protease inhibition, and platelete aggregation activities as viable mechanisms for the anti-inflammatory effects of the methanol extract of Rauvolfia vomitoria Afzel leaf (MERVL).
Methodology: The anti-inflammatory effect of (MERVL) was ascertained using the phosphate lipaseA2, membrane stabilization model, albumin denaturation, protease inhibitor, assay.
Place and Duration of the Study: Department of Pharmacology Lab, Enugu State of University of Science and Technology Agbani Nigeria, between March 2021 and August 2021.
Results: MERVL inhibited hypotonicity-induced haemolysis by 27.14, 41.10 and 65.70%, at the concentration of 0.4, 0.8 and, 1.0 mg/mL respectively.The highest percentage of inhibition (67.70%) was noticed at the highest concentration of the MERVL. These results were almost analogous to the standard drug (indometacin) used as it exhibited concentration dependent inhibition of albumin denaturation. Protease activity was significantly (P < 0.05) increased at all concentrations which follow the similar tendency as standard drug used. The results showed that MERVL has anti-inflammatory activities.
Keywords: Rauvolfia vomitoria, membrane stabilization, anti-inflammatory, methanol extract