Effect of a Combination of Ethanol Extract of Ficus capensis and Cnidoscolus aconitifolius on Liver and Kidney Function Parameters of Phenylhydrazine-Induced Anemic Rats
Asian Journal of Research in Biochemistry,
Background: Anemia is a condition in which there is a reduced number of red blood cells or haemoglobin and iron concentration in the body thereby leading to a decreased capacity of the blood to carry oxygen to the body tissues.
Objectives: The study was designed to investigate the effect of a combination of ethanol extract of Ficus capensis and Cnidoscolus aconitifolius in phenylhydrazine-induced anemic rats.
Methods: The animals were divided into five groups of five rats each. Group A served as normal control, Group B as anemic control, group C as standard drug control, groups D and E were treated with 200 mg/kg and 400 mg/kg of combined ethanol leaf extract of F. capensis and C. aconitifolius respectively. Phenylhydrazine was administered intraperitoneally at a dose of 20 mg/kg b.w. for two days to induce anemia in rats. The administration of the extract lasted for 14 days after which the animals were sacrificed and blood obtained through cardiac puncture for kidney and liver biomarkers analyses.
Results: Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase, Total bilirubin, Direct bilirubin and Kidney function parameters assayed in the anemic untreated group showed significant increases (p<0.05) compared to the normal rats which may be attributed to toxicity induced by phenylhydrazine. The groups treated with the ethanol extract at a dose of 200 and 400 mg/kg body weight revealed a significant (p<0.05) decrease in the liver function parameters compared to the anemic untreated group. The creatinine and urea levels of the anemic untreated group increased more than the that of the test groups although the increase was not statistically significant.
Conclusion: This study suggests that combined ethanol leaf extract of F. capensis and C. aconitifolius is safe and can be effective in the treatment and management of anemia.
- F. capensis
- C. aconitifolius
- liver enzymes
- kidney function test
How to Cite
WHO. Guidelines on food fortification with micronutrients. Geneva: World Health Organization; 2006.
(Accessed 24th January, 2020)
Ashour TH. Hematinic and anti-anemic effects of thy moquinone against phenylhydrazine induced hemo-lytic anemia in rats. Res. J. Med. Sci. 2014;8(2):67–72.
Kanfer EJ, Nicol BA. Haemoglobin concentration and erythrocyte sedimentation rate in primary care patients. J. R. Soc. Med. 1997;90:16–18.
Oladiji AT, Jacob TO, Yakubu MT. Anti-anemic potentials of aqueous extract of Sorghum bicolor (L) moench stem bark in rats. Journal of Ethnopharmacology. 2007;111:651-656.
Sofowora A. African medicinal plants. Proceeding of Conference. University of Ife Press, Ife Nigeria. 1979;3.
Singh R. Medicinal plants: A review. Journal of Plant Sciences. 2015;3(1):50-55.
Owolabi OJ, Nworgu ZA, Falodun A, Ayinde BA, Nwako CN. Evaluation of tocolytic activity of ethanol extract of the stem bark of Ficus capensis Thumb (Moraceae). Acta Poloniae Pharmaceutica Ñ Drug Research. 2009;66(3):293-296.
Berg CC. Distribution of African taxa of Ficus (Moraceae). [Proc. 12th AETFAT]. MitteilungenAus Dem Institut Fur Allgemeine Botanik Hamburg. 1990;23: 401-405.
Olowokudejo JD, Kadiri AB, Travih VA. An ethnobotanical survey of herbal markets and medicinal plants in Lagos State of Nigeria. Ethnomedicinal Leaflets. 2008;12(8):51-65.
Rowe L. Plant guards: Secret of good health. Valley Morning Star. 1994;4:A12.
Kuti JO, Torres ES. Potential nutritional and health benefits of the spinach. In: J. Jarick (Ed). Progress in New Crops. ASHS Press, Arlington, VA. 1996;516- 520.
Ezeigwe OC, Nwobodo VOG, Enemchukwu BN, Ani ON, Chukwuemeka UV, Ezennaya CF, Okibedi FU, Aroh IL. Lipid profile and its complications in phenylhydrazine-induced anemic rats. Journal of Medicinal Plant Studies. 2019;7(6):161-166.
Rifkind RA, Danon D. Heinz body anemia – An ultrastructural study. I. heinz body formation”. Blood. 1965;25:885-96.
Adamson JW, Longo DL. Hematologic alterations. In: Braumwald E, Fauci AS, Kasper DL, Hauser SL, Longo DL, Jameson JL, Editors. Harrison’s Principles of Internal Medicine. New York: McGraw Hills; 2001.
Waghmare AN, Tembhurne SV, Sakarkar DM. Anti-anaemic potential of Murraya koenigii fruit extracts in phenylhydrazine induced anaemic rats. International Journal of Advances in Psychology Research. 2015;6(5):124-7.
Ndem JI, Otitoju O, Akpanaiabiatu MI, Uboh FE, Uwah AF, Edet OA. Haematoprotective property of Eremomastax speciosa (Hochst.) on experimentally induced anaemic Wistar rats. Annals of Biological Research. 2013;4(6):356-60.
Abstract View: 1575 times
PDF Download: 965 times