Comparative Nephrotoxicity and Hepatotoxicity Effects of Kerosene, Gasoline, Liquefied Petroleum Gas and Biomass Fuel Exposure on Male Albino Rats

Main Article Content

Ude Tochukwu
Meludu Samuel Chukwuemeka
Dioka Chudi Emmanuel
Chikezie Onyebuchi Desmond
Awalu Chimezie Joseph
Ibekailo Sylvester Nnaemeka

Abstract

The effects of Kerosene, gasoline, and liquefied petroleum gas and biomass fuel exposure on biomarkers of kidney and liver were investigated in male wistar rats. Fifty adult male wistar rats were randomly assigned to five groups of ten animals each. Rats in group A served as control (exposed to fresh air). Group B, C, D and E were exposed to inhalation of kerosene, gasoline, liquefied petroleum gas and biomass fuel (wood smoke) respectively. All the exposures were done using whole body exposure chambers 70 cm x 60 cm x 60 cm measurement for six weeks, 6 days per week. Five millilitres of blood sample were collected and serum extracted at the end of six weeks. The serum concentration of urea, creatinine, uric acid and activities AST, ALT, γGT were determined using Cobas reagent kits manufactured by Roche Diagnostics GmbH, Sandhofer Strasse 116, D-68305 Mannheim, Germany. Values were analysed statistically using SPSS version 23.0.  The result shows significant increase in the serum  levels of urea, creatinine and uric acid of test groups relative to control (p<0.05), though the effect appear to be more pronounced with exposure to kerosene, gasoline and biomass fuel. The exposure also led to significant increase in activities of AST, ALT and γGT (p<0.05). These results suggest that repeated exposure to kerosene, gasoline and liquefied petroleum gas and biomass fumes may elicit hepatic and renal toxicity, thereby impairing the normal liver and kidney function.

Keywords:
Kerosene, gasoline, liquefied petroleum gas, biomass fuel, biomarkers, exposure.

Article Details

How to Cite
Tochukwu, U., Chukwuemeka, M. S., Emmanuel, D. C., Desmond, C. O., Joseph, A. C., & Nnaemeka, I. S. (2020). Comparative Nephrotoxicity and Hepatotoxicity Effects of Kerosene, Gasoline, Liquefied Petroleum Gas and Biomass Fuel Exposure on Male Albino Rats. Asian Journal of Research in Biochemistry, 7(4), 45-52. https://doi.org/10.9734/ajrb/2020/v7i430147
Section
Original Research Article

References

Uboh FE, Akpanabiatu MI, Eyong EU, Ebong PE, Eka OO. Evaluation of toxicological implications of inhalation exposure to kerosene fumes and petrol fumes in rats. Acta Biol Szeged. 2005;49(3-4):19-22.

Okoye JO, Ude T, Ibekailo SN, Awalu CJ. Histopathological, hemochromatotic, hypercholesterolemic, and androgenic effects of escravos crude oil on the testis in male chinchilla rabbits. Bri. J. Biotechnol. 2014;4(6):649-658.

Hu Z, Wells PG. Modulation of benzo (a) pyrene bioactivation by glucuronidation in lymphocytes and hepatic microsomes from rats with a hereditary deficiency in bilirubin UDP-glucuronosyl transferase. Toxicol. Appl. Pharmacol. 1994;127:306-313.

Nygren J, Cedewal B, Erickson S, Dusinska M, Kolman A. Induction of DNA strand breaks by ethylene oxide in human diploid fibroblasts. Environ. Mol. Mutagen. 1994;24:161-167.

Oluwole O, Arinola GO, Huo D, Olopade CO. Biomass fuel exposure and asthma symptoms among rural school children in Nigeria. J Asthma. 2017b;54:347-356.

Patrick-Iwuanyanwu KC, Onyemaenu CC, Wegwu MO, Ayalogu EO. Hepatotoxic and nephrotoxic effect of kerosene and petrol contaminated diets in wistar albino rats. J. Environ. Toxicol. 2011;5(1):49-57.

Chilcott RP, Chapd HQ. Summary of health effects of diesel. Sci. Total Environ. 2007;20:129-138.

Eyong EU, Umoh IB, Ebong PE, Eteng MU, Antai AB, Akpa AO. Haematoxic effects following ingestion of Nigerian crude oil and crude oil polluted shellfish by rats. Nig. J. Physiol. Sci. 2004;19:1-6.

Okoro AM, Ani EJ, Ibu JO, Akpogomeh BA. Effect of petroleum products inhalation on some haematological indices of fuel attendants in Calaber Metropolis, Nigeria. Nig J Physiol Sci. 2006;21:71–75.

Sirdah MM, Allaham NA, El Madhoun RA. Possible health effects of liquefied petroleum gas on workers at filling and distribution stations of Gaza governorates. EMHJ. 2013;19(3):289-294.

Udonwa NE, Uko EK, Ikpeme BM, Ibanga IA, Okon BO. Exposure of petrol station attendants and auto mechanics to premium motor spirit fumes in Calabar, Nigeria. J En-viron Public Health. 2009;281876.

Akintunde JK, Abioye JB, Ebinama ON. Potential protective effects of Naringin on Oculo-pulmonary injury induced by PM10 (Wood Smoke) exposure by modulation of oxidative damage and acetylcholine esterase activity in a rat model. Curr Ther Res Clin Exp. 2020;92:100586.

Bergmeyer HU, Scheibe P, Wahlefeld AW. Methods for aspartate and alanine amino transferase. Clin Chem. 1979;125:1487.

Persijn JP, Van der Slik W. A new method for the determination of gamma-glutamyltransferase in serum. J. Clin Chem Clin Biochem. 1976;9(14):421-427.

Uboh FE, Akpanbiatu MI, Eteng PMU, Ebong E, Umoh IB. Toxicological effects of exposure to gasoline vapours in male and female rats. Internet J Toxicol. 2008;4:40–45.

Ekpenyong CE, Asuquo EA. Recent advances in occupational and environmental health hazards of workers exposed to gasoline compounds. IJOMEH. 2017;30(1):1-26.

Kurmi OP, Dunster C, Ayres JG, Kelly FJ. Oxidative potential of smoke from burning wood and mixed biomass fuels. Free Radic Res. 2013;47:829–835.

Uhegbu FO, Imo C, Ifeanacho NG. Effect of exposure of male albino rats to kerosene, diesel and petrol on kidney function. Int. Res. J. Environment Sci. 2015;4(11):12-18.

Uboh FE, Akpanbiatu MI, Ndem JI, Alozie Y, Ebong PE. Comparative nephrotoxic effect associated with exposure to diesel and gasoline vapours in rats. J. Toxicol. Environ. Health Sci. 2009;1(4):068-074.

Lum G, Gambino SR. Serum gamma glutamyl transpeptidase activity as an indicator of diseases of liver, pancrease or bone. Clin. Chem. 1972;18:358.

Dere E, Ari F. Effect of benzene on liver function in rats (Rattus norvegicus). Environ Monit Assess. 2009;154:23–27.

George J, Liddle C, Murray M, Byth K, Farrell GC. Pre translational regulation of cytochrom P450 gene is responsible for disease changes of individual P450 enzymes among patients with cirrhosis. Biochem Pharmacol. 1995;9:873–881.

Tanaka T, Uchiumi T, Hinoshita E, Inokuchi A, Toh S, Wada M. The human multi drugs resistance protein 2 gene: Functional characterization of the 5-flanking region and expression in hepatic cells. Hepatology. 1999;30:1507–1512.

Al-Olayan EM, El-Khadragy MF, Aref AM, Othman MS, Kassab RB, Abdel Moneim AE. The potential protective effect of Physalis peruviana L. against carbon tetrachloride induced hepatotoxicity in rats is mediated by suppression of oxidative stress and down regulation of MMP-9 expression. Oxid Med Cell Longev. 2014;381413.

Odunola OA, Uka E, Kazeem A, Akinwumi KA, Gbadegesin MA, Osifeso OO, Ibegbu MD. Exposure of laboratory mice to domestic cooking gas: - Implications for toxicity. Int. J. Environ. Res. Public Health. 2008;5(3):172-176.

Ravnskov U. Experimental glomerulonephritis induced by hydrocarbon exposure: A systemic review. BMC Nephrol. 2005;6:15–18.

Awodele O, Sulayman AA, Akintonwe A. Evaluation of hematological hepatic and renal function of petroleum tanker drivers in Lagos, Nigeria. Afr Health Sci. 2014;14:178–84.

Iyanda AA. Effects of oral and dermal sub-chronic exposure of kerosene on biochemical parameters in male wistar rats. Annu. Res. Rev. Biol. 2013;3(3):188-194.