Enzymatic Activity Profiles at Different Stages of the Development of Bulbils from “Dougou-Won” and "Won-Kpia," Two Varieties of Dioscorea bulbifera
Michel Archange Libra *
Laboratory of Biotechnology and Valorization of Agroresources and Natural Substances, Peleforo Gon Coulibaly University of Korhogo, Ivory Coast.
Fabrice Armel Zoro
Laboratory of Biotechnology and Valorization of Agroresources and Natural Substances, Peleforo Gon Coulibaly University of Korhogo, Ivory Coast.
Apolinaire Kouamé Kouassi
Laboratory of Biotechnology and Valorization of Agroresources and Natural Substances, Peleforo Gon Coulibaly University of Korhogo, Ivory Coast.
Abdoulaye Touré
Laboratory of Biotechnology and Valorization of Agroresources and Natural Substances, Peleforo Gon Coulibaly University of Korhogo, Ivory Coast.
René Yadé Soro
Institute of Agro-Pastoral Management, Peleforo Gon Coulibaly University of Korhogo Box-office 1328 Korhogo, Ivory Coast.
Jean Parfait N’guessan Kouadio
Laboratory of Biocatalysis and Bioprocessing, UFR of Sciences and Food Technologies, University of Nangui Abrogoua Abidjan, PO Box 02 BP 801 Abidjan, Ivory Coast.
Licien Patrice Kouamé
Laboratory of Biocatalysis and Bioprocessing, UFR of Sciences and Food Technologies, University of Nangui Abrogoua Abidjan, PO Box 02 BP 801 Abidjan, Ivory Coast.
*Author to whom correspondence should be addressed.
Abstract
Two varieties of Dioscorea bulbifera (Dougou-won and Won-kpia) were grown to study the enzymatic profile of bulbils during their growth. For each variety, bulbils aged between 1 and 6 months were harvested. After being washed, peeled and cut into strips, the samples were ground in a 0.9% NaCl saline solution. The mixture obtained was centrifuged and the supernatant collected constituted the crude enzyme extract. The experimental conditions consisted of a reaction medium comprising a buffer solution, the enzyme extract and synthetic or natural substrates. The results showed that the bulbils of “Dougou-won” have high levels of polyphenol oxidase (PPO), peroxidase (POD), acid phosphatase and α-amylase, while the bulbils of “Won-kpia” showed high levels of α-D-mannosidase, β-fucosidase and inulase during growth. The study highlighted the enzymatic potential of bulbils of the “Dougou-won” and “Won-kpia” varieties, which could be a valuable source of enzymes for biotechnology and other industrial.
Keywords: Bulbils, “Dougou-won”, “Won-kpia”, Enzymatic activity