Profile of Lipid and Antioxidant Activity Lipids Extracted from Pangasius pangasius: Complementary Analysis
Amir Hossain
Department of Applied Chemistry and Chemical Engineering, Islamic University, Kushtia-7003, Bangladesh.
Md. Mazedul Haque Sachchu
Department of Applied Chemistry and Chemical Engineering, Islamic University, Kushtia-7003, Bangladesh.
Md. Khalid Hossain Shishir
Department of Applied Chemistry and Chemical Engineering, Islamic University, Kushtia-7003, Bangladesh.
Nafis Rahman Sayeem
Department of Applied Chemistry and Chemical Engineering, Islamic University, Kushtia-7003, Bangladesh.
Upoma Podder
Department of Applied Chemistry and Chemical Engineering, Islamic University, Kushtia-7003, Bangladesh.
Md. Rafayet Al-Mahmud
Department of Applied Chemistry and Chemical Engineering, Islamic University, Kushtia-7003, Bangladesh.
Md. Mamun Al Rashid *
Department of Applied Chemistry and Chemical Engineering, Islamic University, Kushtia-7003, Bangladesh.
Rahima Tanbin Tama
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh.
Natasha Nafisa Haque
BCSIR Dhaka Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh.
Aliza Rahman
Department of Chemistry, Pabna University of Science and Technology (PUST), Pabna-6600, Bangladesh.
Md. Ashraful Alam *
Institute of Glass and Ceramic Research and Testing (IGCRT), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Extraction of lipids from Pangasius pangasius employed a unique simple route such as the solvent extraction method which is better useful for extract lipids from fish, cost effective and comparatively easier than others process where ethanol is a diluent and chloroform acts as a solvent. The prime objective of this manuscript is to determine the chemical composition and antioxidant activity of lipids from Pangasius pangasius. The chemical composition determination by Gas Chromatography Mass Spectrometry (GC-MS). The most abundant lipid components were Dodecanoic acid 1,2,3 Propanetriyl ester 86.67% found in GC-MS which have strong bactericidal properties. It is utilized as an algal and plant metabolite and possesses antimicrobial qualities. For determination of the antioxidant activity, the Ultraviolet-Visible Spectrometer (UV) was introduced. The degree of hydrolysis, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity the antioxidant activity can be estimated. The control value of the UV is 1.297 a. u. and the absorption value by sample minimum value is 1.168 a. u. and the maximum value is 1.208 a. u. The inhibition rate maximum of 0.0995% and a minimum of 0.0686% by the human body. The absorption of UV light by the sample is near the control value which indicates lipids contain a little percentage of antioxidant activity. The sample contains a small percentage of 0.33 of L (+) ascorbic acid 2,6 di-hexa-decanoate which is an antioxidant agent that reduces free radicals in the human body. This outcome highlighted its possibility as an origin of bioactive chemicals and expanded our knowledge of its nutritive and therapeutic properties.
Keywords: Antioxidant activity, chloroform, ethanol, Pangasius pangasius, solvent extraction