Therapeutic Potential of Quercetin on Biochemical Deteriorations Induced by Copper Oxide Nanoparticles
Eman R. Youness
Department of Biochemistry, National Research Centre, Cairo, Egypt
Azza F. Arafa
Department of Therapeutic Chemistry, National Research Centre, Cairo, Egypt
Hanan F. Aly *
Department of Therapeutic Chemistry, National Research Centre, Cairo, Egypt
Emad EL-Meligy
Department of Ceramics, National Research Centre, Cairo, Egypt
*Author to whom correspondence should be addressed.
Abstract
Objective: The current research is designed to evaluate copper oxide nanoparitcles (CuO NPs) toxicity on HCT116 in vitro as well as in vivo on liver paraoxanse 1 (PON1 ) activity and serum B-cell lymphoma -2 (BCl2) in rats.
Materials and Methods: The toxicological role of CuO-NPs on the liver was indicated through intraperitoneal injection of 3 and 50 mg/kg of CuO-NPs (size >20 nm) in female rats for 7 days. The effects of NPs were examined by demonstrating serum levels of antiapoptotic marker (BCl2) and antioxidant enzyme PON1 .Flavonoids quercetin (que) was administered orally to intoxicated rats at the dose of 200 mg/kg for 30 consecutive days.
Results: In vitro study showed 100% death of HCT116 cells up to 12.5 ug/ml. The current results declared obvious PON1 inhibition and BCl2 in CuO-NPs intoxicated rats. Also, the results of this study indicated that the two concentrations of copper nanoparticles induced toxicity, while attenuation in antioxidant status and antiapoptotic marker was detected upon treated intoxicated rats with que.
Conclusion: It is evident that these nanoparticles cannot be used for human purposes because of their toxicity caused by oxidative stress implicated in liver toxicity by CuO-NPs. This study also declared that que is an effective free radical scavenger and could consider a potential nutraceutical for liver toxicity.
Keywords: Copper oxide, liver toxicity, nanoparticles, quercetin, PON1, BCl2