In-vitro Biochemical Studies on Organometallic Compounds as Anticancer Agents
F. Z. Mohammed *
Department of Biochemistry, Faculty of Science, Zagazig University, Egypt.
Elsherbiny H. Elsayed
Department of Organic Chemistry, Faculty of Science, Port Said University, Egypt.
Atef E. Abd Elbaky
Department of Biochemistry, Faculty of Pharmacy, Port Said University, Egypt.
H. M. Shalaby
Department of Biochemistry, Faculty of Science, Port Said University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
This study aims to synthesis of copper complexes of 2,3-dihydroxy benzaldehyde thiosemicarbazone (3a,b), followed by evaluating their in vitro anticancer properties. The prepared compounds have been also evaluated for their ability to induce apoptosis. A total number of 80 adult female Swiss albino mice weighing 20-25 gm were divided randomly into 8 groups (10 mice /each group). The in vitro cytotoxic activities of compounds (3a, 3b) were evaluated. Minimum inhibitory concentrations of synthesized compound 3a were found to be 50 μg/mL against MCF-7, HepG2 and PC3 cell lines; also, Minimum inhibitory concentrations of synthesized compound 3b were found to be 50 μg/mL in all cell lines. The apoptotic effect of compounds 3a and 3b were evaluated by measurement Caspase-3 activity and Bcl-2 concentration. The mean values of Caspase-3 activity in positive control were found to be 2.6151 (ng/mL). On the other hand, the mean values of Bcl-2 in positive control were found to be 3.7 (ng/mL). The compounds (3a & 3b) exhibited a significant anticancer activity towards MCF-7, HEPG2 and PC3 cancer cell lines.
Keywords: In vitro anticancer properties, copper complexes, 2,3-dihydroxy benzaldehyde thiosemicarbazone, adult female Swiss albino mice, MCF-7, HepG2 and PC3 cell lines.