https://www.journalajrb.com/index.php/AJRB/issue/feed Asian Journal of Research in Biochemistry 2020-10-23T14:09:03+00:00 Asian Journal of Research in Biochemistry contact@journalajrb.com Open Journal Systems <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> https://www.journalajrb.com/index.php/AJRB/article/view/30143 Antioxidant Potential and the Effects of the Aqueous Extract of Spondias cytherea Fruit Pulp on B16-F10 Melanoma Cell Cycle 2020-10-23T14:09:03+00:00 Feudjio Ndemanou Yolande Mouokeu Raymond Simplice Kamtchueng Monique Odette Teboukeu Gires Kodjio Norbert Ndonkou Jinette Tume B. Christopher tumechrist@yahoo.com <p>Consumption of diets rich in fruits and vegetables bring substantial health benefits. <em>Spondias cytherea</em> is a plant belonging to the family of Anacardiaceae, the fruits are highly consumed in Cameroon and commonly known as “kassimango”. In this work, the antioxidant and cytotoxic activities of the aqueous extract of <em>Spondias cytherea</em> pulp was investigated. The antioxidant capacity was evaluated by DPPH (2,2-diphenyl-1-picryl-hydrazyl) ferric reducing antioxidant power (FRAP) and metal chelating power assays. The Total phenolic and flavonoid contents were determined by Folin-ciocalteu test and aluminum chloride colorimetric test respectively. Selenium, and Zinc contents were evaluated. The effect of <em>S. cytherea</em> fruit aqueous extract on cell cycle of B16-F10 skin cancer cells was investigated using propidium iodide staining. The results revealed that the extract was efficient in reducing Fe<sup>3+ </sup>into Fe<sup>2+ </sup>and its ion chelating power increased with the extract concentration. The IC<sub>50</sub> value for DPPH radical was found to be 96.28 µg/ mL. Total phenolic and flavonoid contents of <em>Spondias cytherea</em> fruits were 649.45 ± 0.71 mg GAE/100 g and 86.69 ± 0.02 mg CE/100 g of dry matter respectively. As far as the tested minerals are concerned, <em>S. cytherea</em> fruit presented 4.48 mg/100 g of zinc and 0.86 µg/100 g of selenium. It was observed that <em>S. cytherea</em> fruit aqueous extract inhibit the proliferation of B16-F10 cancer cells by inducing the cell cycle arrest at Sub G0 phase (apoptotic phase), G0/G1 (proliferation phase) and G2/M phase (mitosis phase). These results indicated that <em>Spondias cytherea</em> fruit have antioxidant and can be used in the management of cancer pending on further researches.</p> 2020-10-06T00:00:00+00:00 ##submission.copyrightStatement## https://www.journalajrb.com/index.php/AJRB/article/view/30144 Protective Effects of Bi-Herbal Formulation of Aqueous Extracts of Vernonia amygdalina and Gongronema latifolium against Gentamicin Induced Nephrotoxicity and Liver Injury in Rats 2020-10-22T04:03:47+00:00 Nwadike Constance nwadikeconstanceimsu@gmail.com Dike-Ndudim Joy Oly-Alawuba Nkeiruka Ezekwe Ahamefula Akanazu Chidimma Aloy-Amadi Oluchi <p>The study was carried out to investigate the protective effects of bi-herbal formulation of aqueous extracts of <em>Vernonia amygdalina</em> and <em>Gongronema latifolium</em> against gentamicin induced nephrotoxicity and liver injury in rats. Forty (n=40) male Wistar albino rats were procured and separated into five groups. Groups I and II served as normal control and experimental control respectively. Groups III to V served as test groups. Rats of experimental control (group II) and test groups were induced with lethal dose of gentamicin. Test groups III and IV were placed on herbal formulation of aqueous extracts of <em>V .amygdalina</em>&nbsp; and <em>G. latifolium</em> respectively, whereas rats in test group V received bi-herbal formulation of aqueous extracts of <em>V. amygdalina</em> and <em>G. latifolium</em>. Nephrotoxic indices such as urea reduced significantly (p&lt;0.05) in test groups (III, IV, and V) when compared to experimental control (group II) and normal control (group I). Creatinine also reduced significantly (p&lt;0.05) in test groups III and V against group II (experimental control), and increased insignificantly (p&gt;0.05) in test group V when compared to normal control (group I). Rats induced with gentamicin had upsurge in liver enzymes indicating possible compromise of hepatocellular integrity but the ameliorating effects of the herbal formulations were seen clearly in test groups in this study as they tried to protect the hepatocellular integrity. The bi-herbal formulation of aqueous extract of 5% v/v each of <em>V. amygdalina</em> and <em>G. latifolium</em> offered the best protection as observed in this study. This study has revealed the protective effects of bi-herbal formulation of aqueous extract of <em>V. amygdalina</em>&nbsp; and <em>G. latifolium</em> against gentamicin induced nephrotoxicity and liver injury in rats.</p> 2020-10-22T00:00:00+00:00 ##submission.copyrightStatement##