Asian Journal of Research in Biochemistry https://www.journalajrb.com/index.php/AJRB <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> en-US contact@journalajrb.com (Asian Journal of Research in Biochemistry) contact@journalajrb.com (Asian Journal of Research in Biochemistry) Wed, 17 Jun 2020 10:04:31 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Biochemical and Histopathological Effect of Detarium microcarpum Stem Bark Extract in Wistar Albino Rats https://www.journalajrb.com/index.php/AJRB/article/view/30123 <p><strong>Aim:</strong> In this study, the methanol stem bark extract of <em>Detarium microcarpum </em>was evaluated for sub-chronic, biochemical and histopathological studies.</p> <p><strong>Methodology: </strong>Sub-chronic toxicity studies was investigated in rats administered with 35, 70 and 140 mg/kg doses of the extract orally for 28 days using standard laboratory procedures after the acute toxicity was carried out.</p> <p><strong>Results:</strong> The median lethal dose (LD<sub>50</sub>) of the extract was calculated to be equal to (≥) 5000 mg/kg body weight in rats orally. Serological studies revealed significant (<em>p</em>&lt;0.05) decrease in Alanine aminotransferase (ALT) at all doses tested, while at 140 mg/kg it caused a significant (<em>p</em>&lt;0.05) increase in Alkaline Phosphatase (ALP). At doses of 70 and 140 mg/kg there was a significant (<em>p</em>&lt;0.05) reduction in creatinine level. Histopathological studies on the liver showed moderate hepatocellular necrosis at doses of 35 and 70 mg/kg, while at 140 mg/kg there was intense hepatocellular necrosis,&nbsp; Kupffer cells and lymphocytes hyperplasia. The Kidney showed intense necrosis of tubules and glomerular necrosis with lymphocytes hyperplasia at all doses tested.&nbsp; The spleen also showed intense lymphocyte hyperplasia at all doses with sinusoidal congestion at the lowest dose of 35 mg/kg. The heart showed slight necrosis of cardiac muscle cells at all doses with blood congestion at 35 and 70mg/kg body weight.</p> <p><strong>Conclusion:</strong> The study indicates that prolong use of the extract in the management of disease conditions may be associated with some adverse effect of some vital organs.</p> Abubakar Bilyamini Mu’azu, Yusif Bello Baba, Adamu Idris Matinja ##submission.copyrightStatement## https://www.journalajrb.com/index.php/AJRB/article/view/30123 Wed, 17 Jun 2020 00:00:00 +0000 Ameliorative Effect of the Methanol Extract of Napoleonae imperialis Leaves against Methotrexate-induced Renal Damage in Albino Rats https://www.journalajrb.com/index.php/AJRB/article/view/30124 <p><strong>Aim:</strong> This study was aimed to evaluate the effect of methanol extract of <em>Napoleonae imperialis leaves</em> against methotrexate renal damage in albino rats.</p> <p><strong>Methodology:</strong> Thirty (30) male albino rats of mean weight 130 g were used for this study. The animals for the study were grouped into five (5) of six (6) rats each. Group A (normal control) received feed and water only and Group B (positive control) was induced with methotrexate without treatment. Test groups (C and D) were orally given 250 mg and 500 mg/kg b.wt of leaves extract, and group E was orally given the extract only (500 mg/kg b.wt) respectively for 14 days. All the rats used in this study were initially subjected to renal damage using 0.5 ml/kg of methotrexate except the normal control group. The rats were sacrificed after 14 days and the blood samples were collected for biochemical analysis,</p> <p><strong>Results:</strong> From the result obtained, there was a significant (p&lt; 0.05) decrease in the groups that received 250 mg/kg and 500 mg/kg b.wt of the plant extract in (Urea, Creatinine and Na<sup>+</sup>), and a significant increase (p&lt; 0.05) in K<sup>+</sup> and Cl<sup>-</sup>. Also there was a significant (p&lt; 0.05) decrease in (Urea, Creatinine, and Na<sup>+</sup>) in comparison with the control groups and the group that received the extract only (500 mg/kg b.wt), and a significant increase (p&lt; 0.05) in (K<sup>+</sup> and Na<sup>+</sup>).</p> <p><strong>Conclusion:</strong> The study indicates that the methanol extract of <em>Napoleonae imperialis leaves </em>may have exerted renal functioning effects in albino rats, and may also be used pharmacologically in the management of organ toxicity.</p> O. J. Mba, G. S. Aloh, E. N. Uhuo ##submission.copyrightStatement## https://www.journalajrb.com/index.php/AJRB/article/view/30124 Wed, 01 Jul 2020 00:00:00 +0000 Anti-cancer Activity of Garcinia mangostana L. and Its Derivatives in Cervical Cancer https://www.journalajrb.com/index.php/AJRB/article/view/30125 <p>The <em>Garcinia mangostana</em> Linn, a medicinal plant commonly used in Southeast Asia. The crude extract and isolated metabolites were used to evaluate its potential anti-cancer activity which was compared to methotrexate (MTX) in cervical cancer cell line. The present study involved in using crude mangosteen (CM), γ-mangostin (γ-M), isopropyl mangostin (IPM) and Di-<em>O</em>-methyl mangostin (DMM) against the standard anti-neoplastic drug MTX. Cell viability and cytotoxicity by lactate dehydrogenase (LDH) were assessed. Analysis of DNA fragmentation and clonogenic assay further supports the anti-cancer activity of the drugs in <em>HeLa </em>cells. The IC<sub>50</sub> value for CM, γ-M, DMM, IPM and MTX were 13.4µg, 34.84µM, 15.57µM 5.3µM and 16.05µM respectively observed in the present study. This study suggests that the <em>G. mangostana </em>and its derivatives have potential anti-cancer activity an <em>in vitro</em> study in cervical cancer.</p> S. V. Kirthanashri, N. Ramesh Kumar, S. Chitra ##submission.copyrightStatement## https://www.journalajrb.com/index.php/AJRB/article/view/30125 Fri, 03 Jul 2020 00:00:00 +0000